Human Lysine-specific demethylase 5A(KDM5A) ELISA kit

Mfr.No.

CSB-EL012141HU

Sensitivity

5.8 pg/mL

Detection Range

23.5 pg/mL-1500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

lysine (K)-specific demethylase 5A, JARID1A, RBBP2, RBP2, Jumonji, AT rich interactive domain 1A (RBBP2-like), Jumonji, AT rich interactive domain 1A (RBP2-like), retinoblastoma binding protein 2, retinoblastoma-binding protein 2

Recovery

The recovery of human HLAMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	93	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HLAMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-97
1:2	Average %	104
	Range %	100-110
1:4	Average %	89
	Range %	84-93
1:8	Average %	96
	Range %	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.482	2.526	2.504	2.320
10	1.870	1.912	1.891	1.707
5	1.145	1.169	1.157	0.973
2.5	0.715	0.693	0.704	0.520
1.25	0.410	0.440	0.425	0.241
0.625	0.290	0.271	0.281	0.097
0.312	0.224	0.237	0.231	0.047
0	0.180	0.188	0.184	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P29375

Applications	ELISA
Research Area	KDM5A

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503228	CSB-EL012141HU	96T	$695	Add to Cart
BHE10503228	CSB-EL012141HU	5x96T	$3336	Add to Cart
BHE10503228	CSB-EL012141HU	10x96T	$6602.50	Add to Cart

Human Lysine-specific demethylase 5A(KDM5A) ELISA kit

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