Human Lys-63-specific Deubiquitinase Brcc36

Mfr.No.

E2075Hu

Alternative Names

BRCA1/BRCA2-containing complex subunit 3;BRCA1/BRCA2-containing complex subunit 36;BRCA1-A complex subunit BRCC36;BRCC3;BRCC36;BRISC complex subunit BRCC36;C6.1A;CXorf53;Lys-63-specific deubiquitinase BRCC36;

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

36072

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.18ng/L

Background

Lys-63-specific deubiquitinase BRCC36 is an enzyme that in humans is encoded by the BRCC3 gene.

Gene ID	79184
UniProt ID	P46736
Antigen	BRCC3

Research Area

Others

Protocol

These standard curves of E2075Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.105	2.062	2.004
	2.018
1600ng/L	1.514	1.47	1.413
	1.427
800ng/L	0.788	0.869	0.812
	0.951
400ng/L	0.43	0.451	0.394
	0.473
200ng/L	0.244	0.271	0.213
	0.298
0ng/L	0.068	0.057	0
	0.046

Precision

We measured random samples of E2075Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1029.94	71.44	6.94
Intra-Assay	2	18	1994.66	91.01	4.56
Intra-Assay	3	18	368.97	17.87	4.84

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103627	E2075Hu	48T	$320		Add to Cart
BHE12103627	E2075Hu	96T	$458		Add to Cart

Human Lys-63-specific Deubiquitinase Brcc36 - BRCC3 ELISA Kit

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