Human Lymphocyte Function Associated Antigen 3

Mfr.No.

E0211Hu

Description

This sandwich kit is for the accurate quantitative detection of human Mannose-binding Protein C (also known as MBL2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Ag3;CD 58;CD antigen CD58;CD58;Lymphocyte function-associated antigen 3;Surface glycoprotein LFA-3

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

28147

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.024ng/ml

Background

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Gene ID	965
UniProt ID	P19256
Antigen	CD58

Research Area

Immunology

Protocol

These standard curves of E0211Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.684	2.564	2.518
	2.443
6ng/ml	1.343	1.391	1.346
	1.439
3ng/ml	0.798	0.849	0.804
	0.899
1.5ng/ml	0.53	0.472	0.427
	0.413
0.75ng/ml	0.192	0.234	0.188
	0.275
0ng/ml	0.031	0.045	0
	0.059

Precision

We measured random samples of E0211Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.2	0.08	7.1
Intra-Assay	2	18	5.9	0.17	2.9
Intra-Assay	3	18	4	0.17	4.2

Storage	2-8°C

Reference	Evaluation of the serum levels of Mannose binding lectin-2, tenascin-C, and total antioxidant capacity in patients with coronary artery disease Journal: J Clin Lab Anal. 2021 Oct;35(10):e23967.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12101931	E0211Hu	96T	$458		Add to Cart
BHE12101931	E0211Hu	48T	$320		Add to Cart

Human Lymphocyte Function Associated Antigen 3 - LFA-3 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Lymphocyte Function Associated Antigen 3 - LFA-3 ELISA Kit

Rate it now..!!