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Home / ELISA Kit

Human Lymphocyte antigen 96 (LY96) ELISA Kit

SKU : BHE13703980

LY96 is involved in binding lipopolysaccharide with TLR 4. It is also known as "MD2". The MD-2 protein appears to associate with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. MD2 did not interact with LY64, but it did appear on cells expressing Toll-like receptor-4 (TLR4). Toll-like receptors, which contain extracellular LRRs and have intracellular regions homologous to the intracellular signaling domain of the interleukin-1 receptor (IL1R), activate nuclear factor kappa-B (NFKB). TLR4 is involved in the response to lipopolysaccharide (LPS) from gram-negative bacteria. Functional analysis showed that expression of MD2 enhanced TLR4-dependent activation of NFKB.

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Mfr.No.	KTE61765
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Lymphocyte antigen 96 (LY96) ELISA Kit employs a two-site sandwich ELISA to quantitate LY96.
Alternative Names	LY96; MD-2; MD2; MD-2 protein; myeloid differentiation protein-2
Detection Method	Colorimetric
Kit Components	Human Lymphocyte antigen 96 microplate Human Lymphocyte antigen 96 standard Human Lymphocyte antigen 96 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Lymphocyte antigen 96 (LY96) ELISA Kit has high sensitivity and excellent specificity for detection of Human LY96. No significant cross-reactivity or interference between Human LY96 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	LY96 is involved in binding lipopolysaccharide with TLR 4. It is also known as "MD2". The MD-2 protein appears to associate with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. MD2 did not interact with LY64, but it did appear on cells expressing Toll-like receptor-4 (TLR4). Toll-like receptors, which contain extracellular LRRs and have intracellular regions homologous to the intracellular signaling domain of the interleukin-1 receptor (IL1R), activate nuclear factor kappa-B (NFKB). TLR4 is involved in the response to lipopolysaccharide (LPS) from gram-negative bacteria. Functional analysis showed that expression of MD2 enhanced TLR4-dependent activation of NFKB.
Notes	This Human Lymphocyte antigen 96 (LY96) ELISA Kit employs a two-site sandwich ELISA to quantitate LY96 in samples. An antibody specific for LY96 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLY96 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY96 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY96 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Lymphocyte antigen 96 (LY96) ELISA Kit employs a two-site sandwich ELISA to quantitate LY96 in samples. An antibody specific for LY96 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLY96 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY96 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY96 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703980	KTE61765	48 T	$339		Add to Cart
BHE13703980	KTE61765	96 T	$589		Add to Cart
BHE13703980	KTE61765	96 Tx5	$2629		Add to Cart
BHE13703980	KTE61765	96 Tx50	$24569		Add to Cart

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