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Home / ELISA Kit

Human Lung specific X protein (LUNX) ELISA Kit

SKU : BHE13703420

PLUNC is the human homolog of murine plunc, and like the mouse gene, is specifically expressed in the upper airways and nasopharyngeal regions. The exact biological function of this gene is not known, however, it has been suggested to be involved in inflammatory responses to irritants in the upper airways. It may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Multiple transcript variants resulting from alternative splicing in the 3' UTR have been detected, but the full-length nature of only two is known.The cDNA encodes a leucine-rich protein of 256 amino acids that is 72% identical to the murine protein. within the human PLUNC family, amino acid sequence identity is low, ranging from 16 to 28%.

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Mfr.No.	KTE61204
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Lung specific X protein (LUNX) ELISA Kit employs a two-site sandwich ELISA to quantitate PLUNC.
Alternative Names	PLUNC; LPLUNC3; LUNX; NASG; SPLUNC1; SPURT; bA49G10.5; OTTHUMP00000030627; ligand-binding protein RYA3; nasopharyngeal carcinoma-related protein; secretory protein in upper respiratory tracts; tracheal epithel
Detection Method	Colorimetric
Kit Components	Human Lung specific X protein microplate Human Lung specific X protein standard Human Lung specific X protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Lung specific X protein (LUNX) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLUNC. No significant cross-reactivity or interference between Human PLUNC and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	PLUNC is the human homolog of murine plunc, and like the mouse gene, is specifically expressed in the upper airways and nasopharyngeal regions. The exact biological function of this gene is not known, however, it has been suggested to be involved in inflammatory responses to irritants in the upper airways. It may also serve as a potential molecular marker for detection of micrometastasis in non-small-cell lung cancer. Multiple transcript variants resulting from alternative splicing in the 3' UTR have been detected, but the full-length nature of only two is known.The cDNA encodes a leucine-rich protein of 256 amino acids that is 72% identical to the murine protein. within the human PLUNC family, amino acid sequence identity is low, ranging from 16 to 28%.
Notes	This Human Lung specific X protein (LUNX) ELISA Kit employs a two-site sandwich ELISA to quantitate PLUNC in samples. An antibody specific for PLUNC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLUNC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLUNC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLUNC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	51297

Applications	ELISA
Applications Notes	This Human Lung specific X protein (LUNX) ELISA Kit employs a two-site sandwich ELISA to quantitate PLUNC in samples. An antibody specific for PLUNC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLUNC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLUNC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLUNC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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