Matrices listed below were spiked with certain level of LTE4 and the recovery rates were calculated by comparing the measured value to the expected amount of LTE4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-102
95
EDTA plasma(n=5)
93-101
98
heparin plasma(n=5)
85-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LTE4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-105%
88-100%
85-103%
EDTA plasma(n=5)
84-97%
86-98%
82-100%
heparin plasma(n=5)
83-94%
80-98%
91-99%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
LTE4 ELISA Kit allows for the in vitro quantitative determination of LTE4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Human LTE4 (Cysteinyl Leukotrienes E4) ELISA Kit