Human LRIG3 ELISA Kit PicoKine®

Mfr.No.

EK1503

Sensitivity

<12 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.019	0.081	0.139	0.267	0.642	1.196	1.767	2.112

Description

Human LRIG3 ELISA Kit PicoKine® (96 Tests). Quantitate Human LRIG3 in cell culture supeatants, serum and plasma (heparin). Sensitivity: 12pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Leucine-rich repeats and immunoglobulin-like domains protein 3;LIG-3;LRIG3;LIG3;UNQ287/PRO326/PRO335;

Molecular Weight

123434 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D28-T807

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human LRIG3

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle membrane ; Single-pass type I membrane protein . Detected in cytoplasmic vesicles when coexpressed with ERBB4. .

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	100	680	1076	92	689	1148
Standard deviation	4.9	46.92	73.16	5.79	48.91	89.54
CV (%)	4.9%	6.9%	6.8%	7.1%%	7.1%	7.8%

Protein Function

May play a role in craniofacial and inner ear morphogenesis during embryonic development. May act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1 (By similarity). .

Background

LRIG3 is a 1,119-amino acid protein that contains a signal peptide. This gene is mapped to chromosome 12q13.2. Northe blot analysis detected a 5.1-kb LRIG3 transcript in all tissues analyzed. Quantitative RT-PCR detected highest expression in stomach and lowest expression in blood. And quantitative RT-PCR of mouse tissues revealed ubiquitous expression, but the patte of expression differed from that in human tissues. LRIG3 may play a role in craniofacial and inner ear morphogenesis during embryonic development. In addition, it may act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	100	108	99	93	100	5.33	5.3%
Sample 2	680	623	576	637	629	37.11	5.8%
Sample 3	1076	1073	1250	1113	1128	72.17	6.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	121227
Gene Name	LRIG3
UniProt ID	Q6UXM1
Protein Name	Leucine-rich repeats and immunoglobulin-like domains protein 3
Tissue Specificity	Widely expressed. .

Applications	ELISA
Research Area	Auditory System,Cancer,Developmental Biology,Embryogenesis,Embryonic Stem Cells,Intracellular,Neuroscience,Sensory System,Stem Cells,Surface Molecules,Tumor Antigens,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001094	EK1503	96 wells/kit	$499		Add to Cart

Human LRIG3 ELISA Kit PicoKine®

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