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Home / ELISA Kit

Human Low molecular-weight protein/proteasome beta-type subunit (LMP7/PSMB9) ELISA Kit

SKU : BHE13703298

Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.
LMP2 isa member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. LMP2 is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit.

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Mfr.No.	KTE61082
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Low molecular-weight protein/proteasome beta-type subunit (LMP7/PSMB9) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMB9.
Alternative Names	PSMB9; DADB-223G19.3; LMP2; MGC70470; PSMB6i; RING12; beta1i; low molecular mass protein 2; macropain chain 7; multicatalytic endopeptidase complex chain 7; proteasome beta 9 subunit; proteasome catalytic subu
Detection Method	Colorimetric
Kit Components	Human Low molecular-weight protein/proteasome beta-type subunit microplate Human Low molecular-weight protein/proteasome beta-type subunit standard Human Low molecular-weight protein/proteasome beta-type subunit detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Low molecular-weight protein/proteasome beta-type subunit (LMP7/PSMB9) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSMB9. No significant cross-reactivity or interference between Human PSMB9 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. LMP2 isa member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. LMP2 is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit.
Notes	This Human Low molecular-weight protein/proteasome beta-type subunit (LMP7/PSMB9) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMB9 in samples. An antibody specific for PSMB9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMB9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMB9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMB9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5698

Applications	ELISA
Applications Notes	This Human Low molecular-weight protein/proteasome beta-type subunit (LMP7/PSMB9) ELISA Kit employs a two-site sandwich ELISA to quantitate PSMB9 in samples. An antibody specific for PSMB9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSMB9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMB9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMB9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703298	KTE61082	48 T	$359		Add to Cart
BHE13703298	KTE61082	96 T	$619		Add to Cart
BHE13703298	KTE61082	96 Tx5	$2809		Add to Cart
BHE13703298	KTE61082	96 Tx50	$26209		Add to Cart

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