Human low-density lipoprotein-receptor-related protein 4(LRP-4)ELISA Kit

Mfr.No.

CSB-E17518h

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

low density lipoprotein receptor-related protein 4, KIAA0816, LRP10, MEGF7, low-density lipoprotein receptor-related protein 4, multiple epidermal growth factor-like domains 7

Recovery

The recovery of mouse SCF/MGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	86	83-89

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SCF/MGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-85
1:2	Average %	100
	Range %	97-103
1:4	Average %	86
	Range %	83-89
1:8	Average %	108
	Range %	104-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	1.804	1.900	1.852	1.735
100	1.195	1.201	1.198	1.081
50	0.731	0.749	0.740	0.623
25	0.448	0.438	0.443	0.326
12.5	0.309	0.306	0.308	0.191
6.25	0.205	0.210	0.208	0.091
3.12	0.151	0.150	0.151	0.034
0	0.118	0.116	0.117	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O75096

Applications	ELISA
Research Area	LRP4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503204	CSB-E17518h	96T	$695	Add to Cart
BHE10503204	CSB-E17518h	5x96T	$3336	Add to Cart
BHE10503204	CSB-E17518h	10x96T	$6602.50	Add to Cart

Human low-density lipoprotein-receptor-related protein 4(LRP-4)ELISA Kit

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