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Home / ELISA Kit

Human Low-density lipoprotein receptor-related protein 2 (LRP2) ELISA Kit

SKU : BHE13703998

LRP2 is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins.

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Mfr.No.	KTE61783
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Low-density lipoprotein receptor-related protein 2 (LRP2) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP2.
Alternative Names	LRP2; DBS; gp330; Heymann nephritis antigen homolog; calcium sensor protein; megalin
Detection Method	Colorimetric
Kit Components	Human Low-density lipoprotein receptor-related protein 2 microplate Human Low-density lipoprotein receptor-related protein 2 standard Human Low-density lipoprotein receptor-related protein 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Low-density lipoprotein receptor-related protein 2 (LRP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRP2. No significant cross-reactivity or interference between Human LRP2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	LRP2 is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins.
Notes	This Human Low-density lipoprotein receptor-related protein 2 (LRP2) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP2 in samples. An antibody specific for LRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4036

Applications	ELISA
Applications Notes	This Human Low-density lipoprotein receptor-related protein 2 (LRP2) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP2 in samples. An antibody specific for LRP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703998	KTE61783	48 T	$339		Add to Cart
BHE13703998	KTE61783	96 T	$589		Add to Cart
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