Human low-density lipoprotein-receptor-related protein 1,LRP-1 ELISA Kit

Human low-density lipoprotein-receptor-related protein 1,LRP-1 ELISA Kit

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Mfr.No.

CSB-E13348h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor), A2MR, APOER, APR, CD91, FLJ16451, IGFBP3R, LRP, MGC88725, TGFBR5, TbetaR-V/LRP-1/IGFBP-3 receptor, alpha-2-macroglobulin receptor, low density lipoprotein-related protein 1, type V tgf-beta receptor

Recovery

The recovery of mouse CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	90
	Range %	86-93
1:40	Average %	103
	Range %	99-106
1:80	Average %	93
	Range %	87-96
1:160	Average %	98
	Range %	93-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.206	2.124	2.165	1.995
500	1.661	1.655	1.658	1.488
250	1.138	1.122	1.130	0.960
125	0.727	0.721	0.724	0.554
62.5	0.424	0.435	0.430	0.260
31.2	0.292	0.305	0.299	0.129
15.6	0.228	0.234	0.231	0.061
0	0.168	0.172	0.170	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q07954

Applications	ELISA
Research Area	LRP1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.