Human low-density lipoprotein-receptor-related protein 1,LRP-1 ELISA Kit

Human low-density lipoprotein-receptor-related protein 1,LRP-1 ELISA Kit

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Mfr.No.

CSB-E13348h

Alternative Names

low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor), A2MR, APOER, APR, CD91, FLJ16451, IGFBP3R, LRP, MGC88725, TGFBR5, TbetaR-V/LRP-1/IGFBP-3 receptor, alpha-2-macroglobulin receptor, low density lipoprotein-related protein 1, type V tgf-beta receptor

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	90
	Range %	86-93
1:40	Average %	103
	Range %	99-106
1:80	Average %	93
	Range %	87-96
1:160	Average %	98
	Range %	93-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.206	2.124	2.165	1.995
500	1.661	1.655	1.658	1.488
250	1.138	1.122	1.130	0.960
125	0.727	0.721	0.724	0.554
62.5	0.424	0.435	0.430	0.260
31.2	0.292	0.305	0.299	0.129
15.6	0.228	0.234	0.231	0.061
0	0.168	0.172	0.170	?

Species

Homo sapiens

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q07954
Antigen	low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)

Applications

ELISA

Research Area

LRP1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503202	CSB-E13348h	96T	$790	Add to Cart
BHE10503202	CSB-E13348h	5x96T	$3792	Add to Cart
BHE10503202	CSB-E13348h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.