Human Low Density Lipoprotein Receptor

Mfr.No.

E1502Hu

Sensitivity

0.092ng/ml

Detection Range

0.2-70ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Low Density Lipoprotein Receptor (also known as LDLR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

LDL receptor;LDLR;Low-density lipoprotein receptor

Molecular Weight

95376

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1502Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	10.5	0.61	5.8
Intra-Assay	2	18	29.1	0.95	3.3
Intra-Assay	3	18	5.5	0.33	6

Background

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

Protocol

These standard curves of E1502Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.319	2.282	2.235
	2.245
20ng/ml	1.223	1.347	1.3
	1.471
10ng/ml	0.816	0.751	0.704
	0.686
5ng/ml	0.466	0.457	0.41
	0.449
2.5ng/ml	0.259	0.261	0.214
	0.264
0ng/ml	0.051	0.047	0
	0.043

Gene ID	3949
UniProt ID	P01130

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103118	E1502Hu	48T	$320		Add to Cart
BHE12103118	E1502Hu	96T	$458		Add to Cart

Human Low Density Lipoprotein Receptor - LDLR ELISA Kit

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