Human Low affinity immunoglobulin gamma Fc region receptor III-B(FCGR3B) ELISA kit

Human Low affinity immunoglobulin gamma Fc region receptor III-B(FCGR3B) ELISA kit

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Mfr.No.

CSB-EL008544HU

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Fc fragment of IgG, low affinity IIIb, receptor (CD16b), CD16, CD16b, FCG3, FCGR3, Fc fragment of IgG, low affinity IIIb, receptor for (CD16), Fc-gamma receptor IIIb (CD 16), low affinity immunoglobulin gamma Fc region receptor III-B

Recovery

The recovery of human MUSK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-99
EDTA plasma (n=4)	98	94-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MUSK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	91-103
1:200	Average %	89
	Range %	84-95
1:400	Average %	95
	Range %	90-99
1:800	Average %	103
	Range %	99-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.675	2.552	2.614	2.510
50	2.226	2.376	2.301	2.197
25	1.672	1.594	1.633	1.529
12.5	0.792	0.816	0.804	0.700
6.25	0.446	0.427	0.437	0.333
3.12	0.236	0.231	0.234	0.130
1.56	0.180	0.189	0.185	0.081
0	0.106	0.102	0.104

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O75015

Applications	ELISA
Research Area	FCGR3B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503198	CSB-EL008544HU	96T	$695	Add to Cart
BHE10503198	CSB-EL008544HU	5x96T	$3336	Add to Cart
BHE10503198	CSB-EL008544HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.