Human Low Affinity Immunoglobulin Gamma Fc Region Receptor 3-B

Mfr.No.

E5598Hu

Alternative Names

CD antigen CD16b;CD16;CD16A;CD16b;FCG3;Fc-gamma RIII;Fc-gamma RIIIb;Fc-gamma RIII-beta;FCGR3;FCGR3A;FCGR3B;FcR10;FcR-10;FCRIII;FCRIIIb;IGFR3;IgG Fc receptor III-1;Low affinity immunoglobulin gamma Fc region receptor III-B

Reactivity

Human

Detection Range

0.5-200ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

26216

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.29ng/mL

Background

Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.

Gene ID	2215
UniProt ID	O75015
Antigen	FCGR3B

Research Area

Immunology

Protocol

These standard curves of E5598Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/mL	2.216	2.308	2.258
	2.399
60ng/mL	1.285	1.312	1.262
	1.339
30ng/mL	0.812	0.847	0.797
	0.883
15ng/mL	0.423	0.431	0.381
	0.439
7.5ng/mL	0.319	0.281	0.231
	0.243
0ng/mL	0.051	0.05	0
	0.05

Precision

We measured random samples of E5598Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	24.46	1.29	5.28
Intra-Assay	2	18	49.48	2.88	5.82
Intra-Assay	3	18	90.19	2.94	3.26

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106960	E5598Hu	48T	$320		Add to Cart
BHE12106960	E5598Hu	96T	$458		Add to Cart

Human Low Affinity Immunoglobulin Gamma Fc Region Receptor 3-B - FCGR3B ELISA Kit

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