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Home / ELISA Kit

Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) ELISA Kit

SKU : BHE13703103

Long-chain-fatty-acid--CoA ligase 1 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation.
In melanocytic cells ACSL1 gene expression may be regulated by MITF.Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.

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Mfr.No.	KTE60887
Description	This Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACSL1.
Alternative Names	ACSL1; ACS1; FACL1; FACL2; LACS; LACS1; LACS2; fatty-acid-Coenzyme A ligase; long-chain 1; fatty-acid-Coenzyme A ligase; long-chain 2; lignoceroyl-CoA synthase; long-chain acyl-CoA synthetase 1; long-chain acy
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Long-chain-fatty-acid--CoA ligase 1 microplate Human Long-chain-fatty-acid--CoA ligase 1 standard Human Long-chain-fatty-acid--CoA ligase 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACSL1. No significant cross-reactivity or interference between Human ACSL1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Long-chain-fatty-acid--CoA ligase 1 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. In melanocytic cells ACSL1 gene expression may be regulated by MITF.Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
Notes	This Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACSL1 in samples. An antibody specific for ACSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	2180
Antigen	ACSL1
Tag	ACSL1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Long-chain-fatty-acid--CoA ligase 1 (ACSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACSL1 in samples. An antibody specific for ACSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13703103	KTE60887	48 T	$339		Add to Cart
BHE13703103	KTE60887	96 T	$589		Add to Cart
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