Human Long-chain-fatty-acid--CoA Ligase 1

Mfr.No.

E5595Hu

Sensitivity

9.26ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ACS1;ACSL 1;ACSL1;Acyl-CoA synthetase 1;Arachidonate--CoA ligase;LACS 1;LACS 2;Long-chain acyl-CoA synthetase 1;Long-chain acyl-CoA synthetase 2;Long-chain fatty acid-CoA ligase 2;Long-chain-fatty-acid--CoA ligase 1;Palmitoyl-CoA ligase 1;Palmitoyl-CoA ligase 2;Phytanate--CoA ligase

Molecular Weight

77943

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5595Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	153.5	6.1	4
Intra-Assay	2	18	1312.4	99.87	7.6
Intra-Assay	3	18	599.2	40.84	6.8

Background

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed: 24269233, PubMed: 22633490, PubMed: 21242590). Preferentially uses palmitoleate, oleate and linoleate (PubMed: 24269233). Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).

Protocol

These standard curves of E5595Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.109	2.122	2.064
	2.136
1200ng/L	1.765	1.794	1.735
	1.822
600ng/L	1.198	1.241	1.183
	1.285
300ng/L	0.722	0.665	0.607
	0.608
150ng/L	0.438	0.42	0.361
	0.401
0ng/L	0.066	0.058	0
	0.05

Gene ID	2180
UniProt ID	P33121

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106957	E5595Hu	48T	$320		Add to Cart
BHE12106957	E5595Hu	96T	$458		Add to Cart

Human Long-chain-fatty-acid--CoA Ligase 1 - ACSL1 ELISA Kit

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