Human Lipoyl Synthase - LIAS ELISA Kit

Mfr.No.

E5587Hu

Alternative Names

HGCLAS;HUSSY-01;LAS;LIAS;LIP1;Lipoate Synthase;Lipoic acid Synthase;Lipoyl Synthase, mitochondrial;Lip-syn;LS;PDHLD

Reactivity

Human

Detection Range

20-4500ng/L

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

41911

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.45ng/L

Background

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Gene ID	11019
UniProt ID	O43766

Research Area

Others

Protocol

These standard curves of E5587Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.271	2.251	2.209
	2.231
1200ng/L	1.541	1.517	1.475
	1.494
600ng/L	0.608	0.688	0.646
	0.768
300ng/L	0.435	0.429	0.387
	0.423
150ng/L	0.206	0.205	0.163
	0.204
0ng/L	0.051	0.042	0
	0.033

Precision

We measured random samples of E5587Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	950.44	49.32	5.19
Intra-Assay	2	18	1234.75	44.44	3.6
Intra-Assay	3	18	541.09	30.77	5.69

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106949	E5587Hu	48T	$320		Add to Cart
BHE12106949	E5587Hu	96T	$458		Add to Cart

Human Lipoyl Synthase - LIAS ELISA Kit

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