Human Lipoxin A4,LXA4 ELISA Kit

Mfr.No.

CSB-E09689h

Sensitivity

0.156 pg/mL

Reactivity

Human

Sample Type

Cell lysates, Serum, Cell culture supernatants, Urine, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Lipoxin A4,LXA4

Recovery

The recovery of mouse C1QA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-99
EDTA plasma (n=4)	93	86-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse C1QA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	95
	Range %	90-110
1:10	Average %	95
	Range %	89-99
1:20	Average %	95
	Range %	87-101
1:40	Average %	96
	Range %	85-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Antigen	Lipoxin A4,LXA4

Applications	ELISA
Research Area	LXA4

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Lipoxin A4 and Neutrophil/Lymphocyte Ratio: A Possible Indicator in Achieved Systemic Risk Factors for Periodontitis Dogan B. et al,Med Sci Monit,2015 Aug 23;21:2485-93. Inverse Relationship between Serum Lipoxin A4 Level and the Risk of Metabolic Syndrome in a Middle-Aged Chinese Population Yu D. et al,PLoS One,2015 10(11):e0142848.

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