Human lipoprotein alpha,Lp-alpha ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08449h

Alternative Names

lipoprotein, Lp(a), AK38, APOA, LP, OTTHUMP00000017543, antiangiogenic AK38 protein, apolipoprotein(a), lipoprotein Lp(a)

Reactivity

Human

Detection Range

62.5 ng/mL-4000 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human EFNB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	84-91
EDTA plasma (n=4)	103	100-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EFNB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	102
	Range %	97-107
1:4	Average %	85
	Range %	82-90
1:8	Average %	99
	Range %	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

53.106 ng/mL

Limit Of Detection

450 nm

Antigen	lipoprotein, Lp(a)

Applications

ELISA

Research Area

LPA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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