Human Lipopolysaccharides,LPS ELISA Kit

Human Lipopolysaccharides,LPS ELISA Kit

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Mfr.No.

CSB-E09945h

Alternative Names

Lipopolysaccharides,LPS

Reactivity

Human

Detection Range

6.25 pg/mL-400 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of bovine CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	97	92-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-98
1:200	Average %	97
	Range %	92-102
1:400	Average %	85
	Range %	81-88
1:800	Average %	89
	Range %	83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
80	0.198	0.186	0.192
40	0.315	0.335	0.325
20	0.486	0.480	0.483
10	0.741	0.782	0.762
5	1.027	1.111	1.069
2.5	1.330	1.426	1.378
1.25	1.546	1.511	1.529
0	2.145	2.263	2.204

Species

Homo sapiens

Sensitivity

1.56 pg/mL

Limit Of Detection

450 nm

Antigen	Lipopolysaccharides,LPS

Applications

ELISA

Research Area

LPS

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The Role of Pathogen-Associated Molecular Patterns in Inflammatory Responses Against Alginate Based Microcapsules Paredes-Juarez GA et al,J Control Release,2013 Sep 16.pii: S0168-3659(13)00797-9. Immunosuppressive Tryptophan Catabolism and Gut Mucosal Dysfunction Following Early HIV Infection Jenabian MA.et al,J Infect Dis.,212(3):355-66. Low-grade endotoxemia and clotting activation in the early phase of pneumonia. Cangemi R.et al,Respirology.,/ NK cells of HIV-1-infected patients with poor CD4+ T-cell reconstitution despite suppressive HAART show reduced IFN-r production and high frequency of autoreactive CD56bright cells Giuliani E.et al,Immunol Lett. ,Aug 19 Changes in intestinal microbiota composition and metabolism coincide with increased intestinal permeability in young adults under prolonged physiologic stress Karl JP.et al,Am J Physiol Gastrointest Liver Physiol.,Mar 23:ajpgi.00066.2017. Gut-Derived Serum Lipopolysaccharide is Associated With Enhanced Risk of Major Adverse Cardiovascular Events in Atrial Fibrillation: Effect of Adherence to Mediterranean Diet Pastori D.et al,J Am Heart Assoc.,Jun 5 Gut-derived endotoxin stimulates factor viii secretion from endothelial cells. implications for hypercoagulability in cirrhosis Roberto C.et al,Journal of Hepatology,Jul 14 Bacterial endotoxin, Staphylococcus aureus nasal carriage and obesity among type two diabetes mellitus patients Sarah L. Hamad.et al,Karbala International Journal of Modern Science,Dec 8 Localization of lipopolysaccharide from Escherichia Coli into human atherosclerotic plaque Carnevale R.et al,Sci Rep,Feb 26 Gut-derived lipopolysaccharides increase post-prandial oxidative stress via Nox2 activation in patients with impaired fasting glucose tolerance: effect of extra-virgin olive oil Roberto Carnevale.et al,European Journal of Nutrition,May 16 Plasma indoleamine 2,3-dioxygenase activity is associated with the size of HIV reservoir in patients receiving antiretroviral therapy Chen J.et al,clinical infectious diseases,August 10

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.