Human Lipolysis-stimulated lipoprotein receptor(LSR) ELISA kit

Human Lipolysis-stimulated lipoprotein receptor(LSR) ELISA kit

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Mfr.No.

CSB-EL013215HU

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

lipolysis stimulated lipoprotein receptor, ILDR3, LISCH7, MGC10659, MGC48312, MGC48503, LISCH protein, immunoglobulin-like domain containing receptor 3, lipolysis-stimulated receptor, lipolysis-stimulated remnant, liver-specific bHLH-Zip transcr

Recovery

The recovery of human Klotho spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	97	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	86-92
1:2	Average %	98
	Range %	91-105
1:4	Average %	96
	Range %	92-102
1:8	Average %	93
	Range %	86-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.491	2.452	2.472	2.350
5	2.104	2.076	2.090	1.968
2.5	1.504	1.562	1.533	1.411
1.25	0.869	0.931	0.900	0.778
0.625	0.494	0.507	0.501	0.379
0.312	0.292	0.279	0.286	0.164
0.156	0.185	0.179	0.182	0.060
0	0.126	0.117	0.122	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q86X29

Applications	ELISA
Research Area	LSR

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503174	CSB-EL013215HU	96T	$695	Add to Cart
BHE10503174	CSB-EL013215HU	5x96T	$3336	Add to Cart
BHE10503174	CSB-EL013215HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.