Human Lipocalin-1(LCN1) ELISA kit

Human Lipocalin-1(LCN1) ELISA kit

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Mfr.No.

CSB-EL012810HU

Sensitivity

0.39 ng/mL

Reactivity

Human

Sample Type

Saliva, Serum, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

lipocalin 1 (tear prealbumin), MGC71975, PMFA, TP, VEGP, OTTHUMP00000022539, Von Ebner gland protein, lipocalin 1, lipocalin 1 (protein migrating faster than albumin, tear prealbumin), lipocalin 1-like 2, tear lipocalin

Recovery

The recovery of bovine LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	90	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	90
	Range %	87-94
1:4	Average %	89
	Range %	85-94
1:8	Average %	100
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
50	0.262	0.253	0.258
25	0.520	0.512	0.516
12.5	0.831	0.877	0.854
6.25	1.716	1.668	1.692
3.12	2.439	2.519	2.479

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P31025
Antigen	lipocalin 1 (tear prealbumin)

Applications	ELISA
Research Area	LCN1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503172	CSB-EL012810HU	96T	$695	Add to Cart
BHE10503172	CSB-EL012810HU	5x96T	$3336	Add to Cart
BHE10503172	CSB-EL012810HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.