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Home / ELISA Kit

Human Leukosialin (SPN) ELISA Kit

SKU : BHE13702546

Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.The nucleotide sequence of the 1.7-kb clone showed that the sialophorin transcript has a 3-prime-noncoding region of 587 nucleotides, is polyadenylated, and has the AATAAA polyadenylation signal. The derived sequence of 341 amino acids showed that the sialophorin protein has 3 domains: an extracellular domain of 195 amino acids, a hydrophobic transmembrane domain of 23 amino acids, and a C-terminal intracellular domain of 123 amino acids which contains a number of sites that might be phosphorylated by protein kinase C.

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Mfr.No.	KTE60329
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Leukosialin (SPN) ELISA Kit employs a two-site sandwich ELISA to quantitate SPN.
Alternative Names	SPN; CD43; GPL115; LSN; leukosialin; sialophorin (gpL115; leukosialin; CD43); sialophorin (leukosialin; CD43)
Detection Method	Colorimetric
Kit Components	Human Leukosialin microplate Human Leukosialin standard Human Leukosialin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Leukosialin (SPN) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPN. No significant cross-reactivity or interference between Human SPN and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.The nucleotide sequence of the 1.7-kb clone showed that the sialophorin transcript has a 3-prime-noncoding region of 587 nucleotides, is polyadenylated, and has the AATAAA polyadenylation signal. The derived sequence of 341 amino acids showed that the sialophorin protein has 3 domains: an extracellular domain of 195 amino acids, a hydrophobic transmembrane domain of 23 amino acids, and a C-terminal intracellular domain of 123 amino acids which contains a number of sites that might be phosphorylated by protein kinase C.
Notes	This Human Leukosialin (SPN) ELISA Kit employs a two-site sandwich ELISA to quantitate SPN in samples. An antibody specific for SPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6693

Applications	ELISA
Applications Notes	This Human Leukosialin (SPN) ELISA Kit employs a two-site sandwich ELISA to quantitate SPN in samples. An antibody specific for SPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13702546	KTE60329	48 T	$339		Add to Cart
BHE13702546	KTE60329	96 T	$589		Add to Cart
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