Human Leukocyte Immunoglobulin-like Receptor Subfamily A Member 3

Mfr.No.

E5574Hu

Alternative Names

CD antigen CD85e;CD85 antigen-like family member E;ILT-6;Immunoglobulin-like transcript 6;Leukocyte immunoglobulin-like receptor 4;Leukocyte immunoglobulin-like receptor subfamily A member 3;LILRA 3;LILRA3;LIR-4;Monocyte inhibitory receptor HM43/HM31

Reactivity

Human

Detection Range

0.05-20ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

47472

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.023ng/mL

Background

Acts as soluble receptor for class I MHC antigens. Binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.

Gene ID	11026
UniProt ID	Q8N6C8
Antigen	LILRA3

Research Area

Immunology

Protocol

These standard curves of E5574Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.054	2.191	2.129
	2.327
6ng/mL	1.332	1.426	1.364
	1.52
3ng/mL	0.953	0.891	0.829
	0.829
1.5ng/mL	0.409	0.459	0.397
	0.509
0.75ng/mL	0.258	0.27	0.208
	0.282
0ng/mL	0.071	0.062	0
	0.053

Precision

We measured random samples of E5574Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.2	0.08	6.6
Intra-Assay	2	18	5.8	0.24	4.2
Intra-Assay	3	18	2.8	0.12	4.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106936	E5574Hu	48T	$320		Add to Cart
BHE12106936	E5574Hu	96T	$458		Add to Cart

Human Leukocyte Immunoglobulin-like Receptor Subfamily A Member 3 - LILRA3 ELISA Kit

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