Human Leukocyte immunoglobulin-like receptor subfamily A member 3(LILRA3) ELISA kit

Human Leukocyte immunoglobulin-like receptor subfamily A member 3(LILRA3) ELISA kit

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Mfr.No.

CSB-EL012935HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member 3, CD85E, HM31, HM43, ILT6, LIR-4, LIR4, e3, leucocyte immunoglobulin-like receptor, leukocyte immunoglobulin-like receptor 4, leukocyte immunoglobulin-like receptor A3

Recovery

The recovery of human KDM5A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	95	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human KDM5A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-104
1:2	Average %	90
	Range %	86-94
1:4	Average %	97
	Range %	92-101
1:8	Average %	87
	Range %	83-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.367	2.272	2.320	2.229
750	2.052	2.006	2.029	1.938
375	1.593	1.534	1.564	1.473
187.5	1.194	1.109	1.152	1.061
94	0.689	0.674	0.682	0.591
47	0.319	0.341	0.330	0.239
23.5	0.169	0.175	0.172	0.081
0	0.087	0.094	0.091	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q8N6C8

Applications	ELISA
Research Area	LILRA3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503148	CSB-EL012935HU	96T	$695	Add to Cart
BHE10503148	CSB-EL012935HU	5x96T	$3336	Add to Cart
BHE10503148	CSB-EL012935HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.