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Home / ELISA Kit

Human Leucine-rich repeat-containing protein 4B (LRRC4B) ELISA Kit

SKU : BHE13703993

LRRC4B is a synaptic adhesion protein. It regulates the formation of excitatory synapses. The trans-synaptic adhesion between LRRC4B and LAR regulates excitatory synapse formation in a bidirectional manner.
LRRC4 are post-synaptic adhesion molecules of the LRR protein family that induce excitatory synapse formation. Mature LRRC4 family members are type I transmembrane proteins with an extracellular region that contains nine LRRs, a C2-type Ig-like domain, and a Thr-rich segment. LRRC4 family members share ~55% aa identity, but each recognizes different ligands. LRRC4 and LRRC4C recognize Netrin-G2 and Netrin-G3, respectively, while LRRC4B recognizes the receptor tyrosine phosphatases LAR/PTPRF, PTPR delta and PTPR sigma.

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Mfr.No.	KTE61778
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Leucine-rich repeat-containing protein 4B (LRRC4B) ELISA Kit employs a two-site sandwich ELISA to quantitate LRRC4B.
Alternative Names	LRRC4B; DKFZp761A179; HSM; LRIG4; leucine-rich repeats and immunoglobulin-like domains 4
Detection Method	Colorimetric
Kit Components	Human Leucine-rich repeat-containing protein 4B microplate Human Leucine-rich repeat-containing protein 4B standard Human Leucine-rich repeat-containing protein 4B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Leucine-rich repeat-containing protein 4B (LRRC4B) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRRC4B. No significant cross-reactivity or interference between Human LRRC4B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	LRRC4B is a synaptic adhesion protein. It regulates the formation of excitatory synapses. The trans-synaptic adhesion between LRRC4B and LAR regulates excitatory synapse formation in a bidirectional manner. LRRC4 are post-synaptic adhesion molecules of the LRR protein family that induce excitatory synapse formation. Mature LRRC4 family members are type I transmembrane proteins with an extracellular region that contains nine LRRs, a C2-type Ig-like domain, and a Thr-rich segment. LRRC4 family members share ~55% aa identity, but each recognizes different ligands. LRRC4 and LRRC4C recognize Netrin-G2 and Netrin-G3, respectively, while LRRC4B recognizes the receptor tyrosine phosphatases LAR/PTPRF, PTPR delta and PTPR sigma.
Notes	This Human Leucine-rich repeat-containing protein 4B (LRRC4B) ELISA Kit employs a two-site sandwich ELISA to quantitate LRRC4B in samples. An antibody specific for LRRC4B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRRC4B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRRC4B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRRC4B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	94030

Applications	ELISA
Applications Notes	This Human Leucine-rich repeat-containing protein 4B (LRRC4B) ELISA Kit employs a two-site sandwich ELISA to quantitate LRRC4B in samples. An antibody specific for LRRC4B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRRC4B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRRC4B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRRC4B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703993	KTE61778	48 T	$339		Add to Cart
BHE13703993	KTE61778	96 T	$589		Add to Cart
BHE13703993	KTE61778	96 Tx5	$2629		Add to Cart
BHE13703993	KTE61778	96 Tx50	$24569		Add to Cart

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