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Home / ELISA Kit

Human LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1) ELISA Kit

SKU : BHE13704043

LETMD1 is involved in tumorigenesis and may function as a negative regulator of the TP53/p53. It is found in kidney, liver, skeletal muscle, heart and brain and is over-expressed in various tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon and uterine cervix. There are six isoforms.HCCR-2 that was overexpressed in various human tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon, and uterine cervix. Ectopic expression of HCCR-2 resulted in direct tumorigenic conversions of NIH/3T3 and Rat1 fibroblasts. HCCR-2 probably represents a new oncogene that is related to tumorigenesis, functioning as a negative regulator of the p53 tumor suppressor.

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Mfr.No.	KTE61828
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1) ELISA Kit employs a two-site sandwich ELISA to quantitate LETM1.
Alternative Names	LETM1; LETM1 and EF-hand domain-containing protein 1; mitochondrial
Detection Method	Colorimetric
Kit Components	Human LETM1 and EF-hand domain-containing protein 1, mitochondrial microplate Human LETM1 and EF-hand domain-containing protein 1, mitochondrial standard Human LETM1 and EF-hand domain-containing protein 1, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LETM1. No significant cross-reactivity or interference between Human LETM1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	LETMD1 is involved in tumorigenesis and may function as a negative regulator of the TP53/p53. It is found in kidney, liver, skeletal muscle, heart and brain and is over-expressed in various tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon and uterine cervix. There are six isoforms.HCCR-2 that was overexpressed in various human tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon, and uterine cervix. Ectopic expression of HCCR-2 resulted in direct tumorigenic conversions of NIH/3T3 and Rat1 fibroblasts. HCCR-2 probably represents a new oncogene that is related to tumorigenesis, functioning as a negative regulator of the p53 tumor suppressor.
Notes	This Human LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1) ELISA Kit employs a two-site sandwich ELISA to quantitate LETM1 in samples. An antibody specific for LETM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLETM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LETM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LETM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	3954

Applications	ELISA
Applications Notes	This Human LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1) ELISA Kit employs a two-site sandwich ELISA to quantitate LETM1 in samples. An antibody specific for LETM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLETM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LETM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LETM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704043	KTE61828	48 T	$339		Add to Cart
BHE13704043	KTE61828	96 T	$589		Add to Cart
BHE13704043	KTE61828	96 Tx5	$2629		Add to Cart
BHE13704043	KTE61828	96 Tx50	$24569		Add to Cart

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