Human left-right determination factor 2(LEFTY2) ELISA Kit

Mfr.No.

CSB-E13590h

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

left-right determination factor 2, EBAF, LEFTA, LEFTYA, MGC46222, TGFB4, endometrial bleeding associated factor, endometrial bleeding associated factor (left-right determination, factor A; transforming growth factor beta superfamily),, left-right determination factor 2, EBAF, LEFTA, LEFTYA, MGC46222, TGFB4, endometrial bleeding associated factor, endometrial bleeding associated factor (left-right determination, factor A; transforming growth factor beta superfamily),

Recovery

The recovery of human C1QTNF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	97	93-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C1QTNF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	95
	Range %	91-99
1:10	Average %	103
	Range %	98-106
1:20	Average %	110
	Range %	106-114
1:40	Average %	92
	Range %	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.532	2.604	2.568	2.382
5	2.059	1.987	2.023	1.837
2.5	1.307	1.337	1.322	1.136
1.25	0.773	0.768	0.771	0.585
0.625	0.503	0.512	0.508	0.322
0.312	0.307	0.314	0.311	0.125
0.156	0.247	0.257	0.252	0.066
0	0.185	0.187	0.186	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	O00292

Applications	ELISA
Research Area	LEFTY2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503114	CSB-E13590h	96T	$790	Add to Cart
BHE10503114	CSB-E13590h	5x96T	$3792	Add to Cart
BHE10503114	CSB-E13590h	10x96T	$7505	Add to Cart

Human left-right determination factor 2(LEFTY2) ELISA Kit

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