Human lectin-galactose binding-soluble 3,Lgals3 ELISA Kit

Human lectin-galactose binding-soluble 3,Lgals3 ELISA Kit

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Mfr.No.

CSB-E11807h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

lectin, galactoside-binding, soluble, 3, CBP35, GAL3, GALBP, GALIG, LGALS2, MAC2, IgE-binding protein, MAC-2 antigen, carbohydrate-binding protein 35, galactose-specific lectin 3, galectin 3, galectin-3 internal, laminin-binding protein

Recovery

The recovery of mouse vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	93
	Range %	88-98
1:200	Average %	97
	Range %	92-101
1:400	Average %	99
	Range %	95-104
1:800	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.323	2.451	2.387	2.293
25	1.645	1.788	1.717	1.623
12.5	1.067	1.021	1.044	0.950
6.25	0.602	0.592	0.597	0.503
3.12	0.283	0.302	0.293	0.199
1.56	0.245	0.235	0.240	0.146
0.78	0.166	0.169	0.168	0.074
0	0.092	0.096	0.094	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P17931

Applications	ELISA
Research Area	LGALS3

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.