Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of LCT and the recovery rates were calculated by comparing the measured value to the expected amount of LCT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
93
EDTA plasma(n=5)
81-99
93
heparin plasma(n=5)
85-102
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LCT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
80-96%
83-98%
81-103%
EDTA plasma(n=5)
91-101%
91-101%
90-100%
heparin plasma(n=5)
90-97%
91-95%
90-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P09848
Applications
ELISA
Applications Notes
LCT ELISA Kit allows for the in vitro quantitative determination of LCT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human LCT (Lactase-phlorizin hydrolase) ELISA Kit