Human Laminin subunit alpha-4(LAMA4) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL012728HU

Alternative Names

laminin, alpha 4, RP1-142L7.4, DKFZp686D23145, LAMA3, LAMA4*-1, laminin alpha 4 chain

Reactivity

Human

Detection Range

23.5 pg/mL-1500 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	92	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	93-103
1:200	Average %	104
	Range %	100-108
1:400	Average %	87
	Range %	80-94
1:800	Average %	97
	Range %	94-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

5.86 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q16363
Antigen	laminin, alpha 4

Applications

ELISA

Research Area

LAMA4

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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