Human Laminin subunit alpha-3(LAMA3) ELISA kit

Human Laminin subunit alpha-3(LAMA3) ELISA kit

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Mfr.No.

CSB-EL012727HU

Sensitivity

2.35 ng/mL

Detection Range

9.4 ng/mL-600 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

laminin, alpha 3, BM600, E170, LAMNA, LOCS, lama3a, BM600 150kD subunit, epiligrin 170 kda subunit, epiligrin alpha 3 subunit, kalinin 165kD subunit, laminin alpha 3 subunit, laminin, alpha 3 (nicein (150kD), kalinin (165

Recovery

The recovery of mouse LDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	98	93-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	87
	Range %	83-90
1:200	Average %	85
	Range %	81-90
1:400	Average %	95
	Range %	92-98
1:800	Average %	100
	Range %	98-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
500	0.157	0.159	0.158
250	0.219	0.239	0.229
125	0.334	0.315	0.325
62.5	0.537	0.523	0.530
31.2	0.875	0.898	0.887
15.6	1.199	1.187	1.193
7.8	1.558	1.578	1.568
0	2.503	2.534	2.519

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q16787

Applications	ELISA
Research Area	LAMA3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503097	CSB-EL012727HU	96T	$695	Add to Cart
BHE10503097	CSB-EL012727HU	5x96T	$3336	Add to Cart
BHE10503097	CSB-EL012727HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.