Human lamin A/C (LMNA) ELISA kit

Human lamin A/C (LMNA) ELISA kit

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Mfr.No.

CSB-EL013003HU

Alternative Names

lamin A/C, RP11-54H19.1, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, PRO1, 70 kDa lamin, OTTHUMP00000015843

Reactivity

Human

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	101-113
EDTA plasma (n=4)	94	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	98-107
1:2	Average %	96
	Range %	91-100
1:4	Average %	90
	Range %	83-97
1:8	Average %	99
	Range %	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.954	2.911	2.933	2.783
50	1.933	1.973	1.953	1.803
25	1.365	1.480	1.423	1.273
12.5	0.894	0.885	0.890	0.740
6.25	0.558	0.599	0.579	0.429
3.12	0.313	0.328	0.321	0.171
1.56	0.222	0.230	0.226	0.076
0	0.145	0.154	0.150

Species

Homo sapiens

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P02545
Antigen	Lamin A/C

Applications

ELISA

Research Area

LMNA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503092	CSB-EL013003HU	96T	$695	Add to Cart
BHE10503092	CSB-EL013003HU	5x96T	$3336	Add to Cart
BHE10503092	CSB-EL013003HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.