Human lambda immunoglobulin light chain,lambda-IgLC ELISA Kit
BHE10503091
This Human C4b ELISA Kit was designed for the quantitative measurement of Human C4b protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL .
The recovery of human C4b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
96
89-98
EDTA plasma (n=4)
98
90-105
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C4b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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Sample
Serum(n=4)
1:200
Average %
84
Range %
80-94
1:400
Average %
97
Range %
91-102
1:800
Average %
98
Range %
92-105
1:1600
Average %
92
Range %
86-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.