Human L-Selectin - SELL ELISA Kit

Mfr.No.

E5602Hu

Alternative Names

CD antigen CD62L;CD62 antigen-like family member L;gp90-MEL;LAM-1;LECAM1;Leukocyte adhesion molecule 1;Leukocyte surface antigen Leu-8;Leukocyte-endothelial cell adhesion molecule 1;L-selectin;Lymph node homing receptor;SELL;TQ1

Reactivity

Human

Detection Range

0.3-90ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

42187

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.175ng/mL

Background

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed: 12403782, PubMed: 28489325, PubMed: 28011641). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed: 12403782, PubMed: 28011641).

Gene ID	6402
UniProt ID	P14151
Antigen	SELL

Research Area

Stem Cells

Protocol

These standard curves of E5602Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.413	2.307	2.255
	2.201
24ng/mL	1.48	1.415	1.363
	1.351
12ng/mL	0.718	0.702	0.649
	0.686
6ng/mL	0.467	0.489	0.436
	0.51
3ng/mL	0.315	0.311	0.258
	0.306
0ng/mL	0.065	0.052	0
	0.04

Precision

We measured random samples of E5602Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.7	0.53	6.9
Intra-Assay	2	18	18.4	1.21	6.6
Intra-Assay	3	18	35.9	1.41	3.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106964	E5602Hu	48T	$320		Add to Cart
BHE12106964	E5602Hu	96T	$458		Add to Cart

Human L-Selectin - SELL ELISA Kit

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