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Home / ELISA Kit

Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit

SKU : BHE13704761

Lactate dehydrogenase (LDH or LD) is an enzyme found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. LDH exist in four distinct enzyme classes. This article is specifically about the NAD(P)-dependent L-lactate dehydrogenase. Other LDHs act on D-lactate and/or are dependent on cytochrome c: D-lactate dehydrogenase (cytochrome)) and L-lactate (L-lactate dehydrogenase (cytochrome)). LDH is expressed extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease such as heart failure.

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Mfr.No.	KTE62554
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit employs a two-site sandwich ELISA to quantitate L-LDH.
Alternative Names	L-LDH
Detection Method	Colorimetric
Kit Components	Human L-Lactate Dehydrogenase microplate Human L-Lactate Dehydrogenase standard Human L-Lactate Dehydrogenase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit has high sensitivity and excellent specificity for detection of Human L-LDH. No significant cross-reactivity or interference between Human L-LDH and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Lactate dehydrogenase (LDH or LD) is an enzyme found in nearly all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back. A dehydrogenase is an enzyme that transfers a hydride from one molecule to another. LDH exist in four distinct enzyme classes. This article is specifically about the NAD(P)-dependent L-lactate dehydrogenase. Other LDHs act on D-lactate and/or are dependent on cytochrome c: D-lactate dehydrogenase (cytochrome)) and L-lactate (L-lactate dehydrogenase (cytochrome)). LDH is expressed extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease such as heart failure.
Notes	This Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit employs a two-site sandwich ELISA to quantitate L-LDH in samples. An antibody specific for L-LDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyL-LDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for L-LDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of L-LDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human L-Lactate Dehydrogenase (L-LDH) ELISA Kit employs a two-site sandwich ELISA to quantitate L-LDH in samples. An antibody specific for L-LDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyL-LDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for L-LDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of L-LDH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13704761	KTE62554	48 T	$359		Add to Cart
BHE13704761	KTE62554	96 T	$619		Add to Cart
BHE13704761	KTE62554	96 Tx5	$2809		Add to Cart
BHE13704761	KTE62554	96 Tx50	$26209		Add to Cart

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