Human Kynurenine/Alpha-aminoadipate Aminotransferase

Mfr.No.

E5547Hu

Alternative Names

2-aminoadipate aminotransferase;2-aminoadipate transaminase;AadAT;Alpha-aminoadipate aminotransferase;KAT/AadAT;KAT2;KATII;KYAT2;Kynurenine aminotransferase II;Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial;Kynurenine--oxoglutarate aminotransferase II;Kynurenine--oxoglutarate transaminase 2;Kynurenine--oxoglutarate transaminase II;

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

47352

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.26ng/L

Background

Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).

Gene ID	51166
UniProt ID	Q8N5Z0
Antigen	AADAT

Research Area

Others

Protocol

These standard curves of E5547Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.086	2.066	2.023
	2.047
800ng/L	1.234	1.282	1.239
	1.329
400ng/L	0.81	0.774	0.731
	0.738
200ng/L	0.505	0.468	0.425
	0.431
100ng/L	0.196	0.209	0.166
	0.223
0ng/L	0.026	0.043	0
	0.06

Precision

We measured random samples of E5547Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	164.17	10.53	6.42
Intra-Assay	2	18	776.21	27.43	3.53
Intra-Assay	3	18	570.97	37.75	6.61

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106909	E5547Hu	48T	$320		Add to Cart
BHE12106909	E5547Hu	96T	$458		Add to Cart

Human Kynurenine/Alpha-aminoadipate Aminotransferase - AADAT ELISA Kit

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