Human Kunitz-type protease inhibitor 1(SPINT1) ELISA kit

Mfr.No.

CSB-EL022584HU

Alternative Names

serine peptidase inhibitor, Kunitz type 1, HAI, HAI1, MANSC2, Kunitz-type protease inhibitor 1, hepatocyte growth factor activator inhibitor 1, serine protease inhibitor, Kunitz type 1

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Duration

1-5h

Recovery

The recovery of rat PLAUR/uPAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	83-96
EDTA plasma (n=4)	104	96-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PLAUR/uPAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	103
	Range %	98-106
1:4	Average %	95
	Range %	88-99
1:8	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.113	2.273	2.193	2.072
5	1.643	1.732	1.688	1.567
2.5	1.072	1.119	1.096	0.975
1.25	0.682	0.691	0.687	0.566
0.625	0.404	0.418	0.411	0.290
0.312	0.276	0.289	0.283	0.162
0.156	0.206	0.203	0.205	0.084
0	0.120	0.121	0.121	?

Species

Homo sapiens

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	O43278
Antigen	serine peptidase inhibitor, Kunitz type 1

Applications

ELISA

Research Area

SPINT1

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503083	CSB-EL022584HU	96T	$695	Add to Cart
BHE10503083	CSB-EL022584HU	5x96T	$3336	Add to Cart
BHE10503083	CSB-EL022584HU	10x96T	$6602.50	Add to Cart

Human Kunitz-type protease inhibitor 1(SPINT1) ELISA kit

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