Matrices listed below were spiked with certain level of KIM-1 and the recovery rates were calculated by comparing the measured value to the expected amount of KIM-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-99
94
EDTA plasma(n=5)
88-98
93
heparin plasma(n=5)
85-102
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KIM-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
KIM-1 ELISA Kit allows for the in vitro quantitative determination of KIM-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.