Matrices listed below were spiked with certain level of KHK and the recovery rates were calculated by comparing the measured value to the expected amount of KHK in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-104
100
EDTA plasma(n=5)
87-95
91
heparin plasma(n=5)
87-99
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KHK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
KHK ELISA Kit allows for the in vitro quantitative determination of KHK concentrations in serum, plasma, tissue homogenates and other biological fluids.