Human Keratin - Type Ii Cytoskeletal 2 Epidermal

Mfr.No.

E2973Hu

Alternative Names

CK-2e;Cytokeratin-2e;Epithelial keratin-2e;K2e;Keratin, type II cytoskeletal 2 epidermal;Keratin-2 epidermis;Keratin-2e;KRT 2;KRT2;Type-II keratin Kb2

Reactivity

Human

Detection Range

20-7000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

65433

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

13.14ng/L

Background

Probably contributes to terminal cornification (PubMed: 1380918). Associated with keratinocyte activation, proliferation and keratinization (PubMed: 12598329). Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).

Gene ID	3849
UniProt ID	P35908
Antigen	KRT2

Research Area

Others

Protocol

These standard curves of E2973Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/L	2.395	2.513	2.467
	2.63
2000ng/L	1.326	1.45	1.404
	1.574
1000ng/L	0.795	0.767	0.721
	0.74
500ng/L	0.472	0.457	0.411
	0.441
250ng/L	0.267	0.262	0.217
	0.258
0ng/L	0.036	0.046	0
	0.055

Precision

We measured random samples of E2973Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2399.4	69.82	2.9
Intra-Assay	2	18	1109.1	67.73	6.1
Intra-Assay	3	18	795.1	42.63	5.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104510	E2973Hu	48T	$320		Add to Cart
BHE12104510	E2973Hu	96T	$458		Add to Cart

Human Keratin - Type Ii Cytoskeletal 2 Epidermal - KRT2 ELISA Kit

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