Human Keratin, type II cytoskeletal 1(KRT1) ELISA kit

Mfr.No.

CSB-EL012503HU

Alternative Names

keratin 1, CK1, EHK, EHK1, EPPK, K1, KRT1A, NEPPK, cytokeratin 1, epidermolytic hyperkeratosis 1, hair alpha protein, keratin, type II cytoskeletal 1

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human LDHA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	96	94-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LDHA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

81-92

1:2

Average %

Range %

94-102

1:4

Average %

101

Range %

97-105

1:8

Average %

Range %

88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
2000	2.101	1.952	2.027	1.914
1000	1.676	1.734	1.705	1.592
500	1.252	1.238	1.245	1.132
250	0.768	0.758	0.763	0.650
125	0.566	0.568	0.567	0.454
62.5	0.328	0.321	0.325	0.212
31.25	0.179	0.185	0.182	0.069
0	0.114	0.112	0.113	?

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P04264
Antigen	Keratin 1

Applications

ELISA

Research Area

KRT1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503067	CSB-EL012503HU	96T	$695	Add to Cart
BHE10503067	CSB-EL012503HU	5x96T	$3336	Add to Cart
BHE10503067	CSB-EL012503HU	10x96T	$6602.50	Add to Cart

Human Keratin, type II cytoskeletal 1(KRT1) ELISA kit

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