Human Keratin, type I cytoskeletal 23(KRT23) ELISA kit

Mfr.No.

CSB-EL012539HU

Sensitivity

0.23 ng/mL

Detection Range

0.9 ng/mL-60 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

keratin 23 (histone deacetylase inducible), CK23, DKFZp434G032, HAIK1, K23, MGC26158, cytokeratin 23, histone deacetylase inducible keratin 23, hyperacetylation-inducible type I keratin, keratin 23, keratin, type I cytoskeletal 23, type I intermed

Recovery

The recovery of human LDHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	100-106
EDTA plasma (n=4)	89	85-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LDHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	100
	Range %	97-103
1:4	Average %	87
	Range %	84-90
1:8	Average %	102
	Range %	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
1500	2.184	2.203	2.194	2.038
750	1.706	1.697	1.702	1.546
375	1.215	1.241	1.228	1.072
187.5	0.757	0.738	0.748	0.592
94	0.442	0.468	0.455	0.299
47	0.319	0.298	0.309	0.153
23.5	0.244	0.231	0.238	0.082
0	0.159	0.153	0.156	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9C075

Applications	ELISA
Research Area	KRT23

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503065	CSB-EL012539HU	96T	$695	Add to Cart
BHE10503065	CSB-EL012539HU	5x96T	$3336	Add to Cart
BHE10503065	CSB-EL012539HU	10x96T	$6602.50	Add to Cart

Human Keratin, type I cytoskeletal 23(KRT23) ELISA kit

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