Human Keratin - Type 2 Cytoskeletal 1

Mfr.No.

E3645Hu

Description

This sandwich kit is for the accurate quantitative detection of human Keratin, Type Ⅱ Cytoskeletal 1 (also known as KRT1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

67 kDa cytokeratin;CK-1;Cytokeratin-1;Hair alpha protein;K1;Keratin, type II cytoskeletal 1;Keratin-1;KRT 1;KRT1;Type-II keratin Kb1

Reactivity

Human

Detection Range

0.05-15ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

66039

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.026ng/ml

Background

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

Gene ID	3848
UniProt ID	P04264
Antigen	KRT1

Research Area

Signal Transduction

Protocol

These standard curves of E3645Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	1.866	2.008	1.978
	2.149
4ng/ml	1.049	1.018	0.988
	0.986
2ng/ml	0.4	0.421	0.391
	0.442
1ng/ml	0.293	0.246	0.216
	0.198
0.5ng/ml	0.088	0.127	0.097
	0.166
0ng/ml	0.027	0.03	0
	0.032

Precision

We measured random samples of E3645Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.5	0.11	3.2
Intra-Assay	2	18	5.5	0.12	2.2
Intra-Assay	3	18	2.1	0.1	4.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105119	E3645Hu	48T	$320		Add to Cart
BHE12105119	E3645Hu	96T	$458		Add to Cart

Human Keratin - Type 2 Cytoskeletal 1 - KRT1 ELISA Kit

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