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Home / ELISA Kit

Human Kallistatin (SERPINA4) ELISA Kit

SKU : BHE13702920

Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function.The 1,284-bp cDNA encodes 427 amino acid residues, including a 26-residue signal peptide and a 401-residue mature peptide. The translated amino acid sequence of kallistatin matches the protein sequence and shares 44 to 46% sequence identity with human alpha-1-antichymotrypsin (AACT), alpha-1-antitrypsin (PI), corticosteroid-binding globulin (CBG), thyroxine-binding globulin of serum (TBG), and protein C inhibitor (PCI). All of these except TBG, which is encoded by the X chromosome, are encoded by genes on chromosome 14.

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Mfr.No.	KTE60704
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Kallistatin (SERPINA4) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA4.
Alternative Names	SERPINA4; KAL; KLST; KST; PI4; kallistatin; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 4
Detection Method	Colorimetric
Kit Components	Human Kallistatin microplate Human Kallistatin standard Human Kallistatin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Kallistatin (SERPINA4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINA4. No significant cross-reactivity or interference between Human SERPINA4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function.The 1,284-bp cDNA encodes 427 amino acid residues, including a 26-residue signal peptide and a 401-residue mature peptide. The translated amino acid sequence of kallistatin matches the protein sequence and shares 44 to 46% sequence identity with human alpha-1-antichymotrypsin (AACT), alpha-1-antitrypsin (PI), corticosteroid-binding globulin (CBG), thyroxine-binding globulin of serum (TBG), and protein C inhibitor (PCI). All of these except TBG, which is encoded by the X chromosome, are encoded by genes on chromosome 14.
Notes	This Human Kallistatin (SERPINA4) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA4 in samples. An antibody specific for SERPINA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5267

Applications	ELISA
Applications Notes	This Human Kallistatin (SERPINA4) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA4 in samples. An antibody specific for SERPINA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702920	KTE60704	48 T	$339		Add to Cart
BHE13702920	KTE60704	96 T	$589		Add to Cart
BHE13702920	KTE60704	96 Tx5	$2629		Add to Cart
BHE13702920	KTE60704	96 Tx50	$24569		Add to Cart

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