Human Kallikrein-4(KLK4) ELISA kit

Human Kallikrein-4(KLK4) ELISA kit

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Mfr.No.

CSB-EL012455HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

kallikrein-related peptidase 4, AI2A1, ARM1, EMSP, EMSP1, KLK-L1, MGC116827, MGC116828, PROSTASE, PRSS17, PSTS, androgen-regulated message 1, enamel matrix serine protease 1, kallikrein 4 (prostase, enamel matrix, prostate), kallikre

Recovery

The recovery of rat LCAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-96
EDTA plasma (n=4)	102	92-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat LCAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	102
	Range %	92-107
1:200	Average %	88
	Range %	84-93
1:400	Average %	101
	Range %	94-105
1:800	Average %	102
	Range %	92-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
120	0.075	0.079	0.077
60	0.172	0.178	0.175
30	0.374	0.382	0.378
15	0.634	0.624	0.629
7.5	1.178	1.146	1.162
3.75	1.504	1.533	1.519
1.87	1.673	1.622	1.648
0	2.155	2.078	2.117

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9Y5K2

Applications	ELISA
Research Area	KLK4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503050	CSB-EL012455HU	96T	$695	Add to Cart
BHE10503050	CSB-EL012455HU	5x96T	$3336	Add to Cart
BHE10503050	CSB-EL012455HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.