Human Kallikrein 10,KLK 10 ELISA Kit

Human Kallikrein 10,KLK 10 ELISA Kit

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Mfr.No.

CSB-E10095h

Sensitivity

0.7 ng/mL

Detection Range

1.675 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

kallikrein-related peptidase 10, NES1, PRSSL1, breast normal epithelial cell associated serine protease, kallikrein 10, normal epithelial cell-specific 1, protease, serine-like, 1

Recovery

The recovery of human SDF1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	98	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SDF1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-94
1:2	Average %	97
	Range %	92-103
1:4	Average %	98
	Range %	92-105
1:8	Average %	94
	Range %	86-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.849	2.664	2.757	2.614
4000	2.437	2.291	2.364	2.221
2000	1.677	1.603	1.640	1.497
1000	0.841	0.812	0.827	0.684
500	0.470	0.459	0.465	0.322
250	0.298	0.289	0.294	0.151
125	0.227	0.222	0.225	0.082
0	0.144	0.141	0.143	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O43240

Applications	ELISA
Research Area	KLK10

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10503042	CSB-E10095h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.