Matrices listed below were spiked with certain level of JAK3 and the recovery rates were calculated by comparing the measured value to the expected amount of JAK3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
97
EDTA plasma(n=5)
91-105
97
heparin plasma(n=5)
96-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of JAK3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
JAK3 ELISA Kit allows for the in vitro quantitative determination of JAK3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human JAK3(Tyrosine-protein kinase JAK3) ELISA Kit