Matrices listed below were spiked with certain level of JAK2 and the recovery rates were calculated by comparing the measured value to the expected amount of JAK2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
94
EDTA plasma(n=5)
88-99
93
heparin plasma(n=5)
88-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of JAK2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
JAK2 ELISA Kit allows for the in vitro quantitative determination of JAK2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human JAK2(Tyrosine-protein kinase JAK2) ELISA Kit