Matrices listed below were spiked with certain level of IZUMO1 and the recovery rates were calculated by comparing the measured value to the expected amount of IZUMO1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-101
96
EDTA plasma(n=5)
90-100
95
heparin plasma(n=5)
92-102
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IZUMO1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IZUMO1(Izumo sperm-egg fusion protein 1)/OBF/Oocyte binding/fusion factor/Sperm-specific protein izumo
Notes
For Research Use Only
UniProt ID
Q8IYV9
Antigen
IZUMO1
Applications
ELISA
Applications Notes
IZUMO1 ELISA Kit allows for the in vitro quantitative determination of IZUMO1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human IZUMO1(Izumo sperm-egg fusion protein 1) ELISA Kit